Sequence Identification
The BOLD Identification Engine can be accessed from the header menu. This ID engine will accept a DNA barcode sequence to compare for matches in the library. The Animal ID Engine has several sub-sets of the Barcode Library that can be utilized for matching COI barcodes, including the full library (All Barcode Records on BOLD), and the library of only sequences that have a species name (Species Level Barcode Records). There are also separate tabs for comparing plant and fungi sequences against the respective libraries.
The results page will return a species identification when one is possible. When a species level match cannot be made, BOLD will suggest the most likely matches. Multiple sequences can be run at the same time, and the results page will provide match results for each. On the results page, the user may view further details for the species that are the most likely matches for their queried sequence. For example, the matches can be visualized in hierarchical placement using a tree based identification system. On the resulting taxon ID tree, the query sequence will be marked in red with BOLD sequences appearing in black.
Specimen Identification Results Page
Figure 2-4: BOLD ID Engine results, highlighting the most likely species match and collection location.