Managing sequence alignments and base calls is a critical step in any barcode analysis. To prevent the inconvenience of importing sequences into 3rd party software to analyze and edit, BOLD provides an integrated alignment browser that includes many features popular in other packages. In the newest version of BOLD, the updated alignment browser supports direct editing to the database. Multiple alignment options such as MUSCLE and Kalign algorithms, as well as colourization options, are also available.
In the newest version of BOLD, the updated Alignment Browser supports direct editing to the database. Users can select sequences or single bases then right click to see editing options. Once edited, the entire session can be submitted to upload the edited sequences to their records.
Various distance models and alignment algorithms are available as parameters, as well as options to filter out sequences based on sequence length or sequence issues.
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